Ascidians are known to accumulate extremely high levels of vanadium in their blood cells (up to 350 mM). The branchial sac and the intestine are thought to be the …
The blood of Ascidia nigra: blood cell frequency distribution, morphology, and the distribution and valence of vanadium in living blood cells Biol Bull . 1976 Jun;150(3):426-41. doi: 10.2307/1540683.
The Henze solution, derived originally from the aqueous extraction of pelleted whole blood from the ascidian Phallusia mammillata, was examined using spectral studies and it was found that this solution is formed independently of the vanadium metal ions otherwise associated with the vanadocytes. Abstract The "Henze solution", derived originally from …
To maintain the concentration of sulfate ions, an active transporter must exist in the blood cells, but no such transporter has been reported in vanadium-accumulating ascidians. Methods: We determined the concentration of vanadium and sulfate ions in the blood cells (except for the giant cells) of Ascidia sydneiensis samea. We cloned cDNA for ...
It should be noted that the gel was used as a selective adsorption rather than molecular filtration material. ... curve of dilute blood solutions. J. Physiol., Lond. 71, 229-256. GILBERT K., KUSTIN K. & MCLEOD G. C. (1977) Gel nitra- tion analysis of vanadium in Ascidia nigra blood cell lysate. ... Gel filtration analysis of vanadium in …
The magnetic properties of intact and freeze-dried blood cells of the tunicate Ascidia nigra and of model vanadium(III) and (IV) compounds as polycrystalline solids and in aqueous solution have been measured up to 50 kOe with a SQUID susceptometer. Corrections for the samples' diamagnetism were extr …
Ascidians are known to accumulate extremely high levels of vanadium in their blood cells (up to 350 mM). The branchial sac and the intestine are thought to be the first tissues to contact the outer environment and absorb vanadium ions. The concentration of vanadium in the branchial sac and the intestine of the most vanadium-rich ascidian …
The present study examined which of several types of blood cell contains this substance, using a combination of cell fractionation, chromatographies, and neutron activation …
Specialized blood cells of many tunicates accumulate high concentrations of vanadium and phenolic peptide pigments called tunichromes (TC). In order to determine whether V and TC reside in the same cells, Ascidia nigra and Ascidia ceratodes blood cell subpopulations were isolated by fluorescence-activated cell sorting (flow cytometry) …
The blood cells of ascidians accumulate extremely high levels of the transition metal vanadium. We previously isolated four vanadium-binding proteins (Vanabins 1–4) and a homologous protein (VanabinP) from the vanadium-rich ascidian Ascidia sydneiensis samea.In the present study, we identified cDNAs encoding five different …
10.1002/jcp.1040930217.abs Fractions from a Sephadex gel filtration of homogenized Ascidia nigra blood cell lysate were analyzed for vanadium by atomic …
Centrifuged blood cells of Ascidia nigra (Savigny) were found to contain 1.45 per cent vanadium.4.4. Eggs of Phallusia mamillata Cuvier yielded 0·017 per cent vanadium, dry weight. The ash ...
Gel filtration analysis of vanadium inAscidia nigra blood cell lysate (Q67043448) From Wikidata. ... Description Also known as; English: Gel filtration analysis of vanadium inAscidia nigra blood cell lysate. scientific article published on 01 November 1977. Statements. instance of. scholarly article. 1 reference. stated in. Europe PubMed ...
Fractions from a Sephadex gel filtration of homogenized Ascidia nigra blood cell lysate were analyzed for vanadium by atomic absorption spectroscopy. The results were …
One of the three suborders in ascidians can accumulate a high level of vanadium ions in blood cells. Ascidia gemmata has been reported to accumulate the highest levels of vanadium at 350 mM, which ...
It should be noted that the gel was used as a selective adsorption rather than molecular filtration material. ... curve of dilute blood solutions. J. Physiol., Lond. 71, …
Consumption by the bluehead wrasse, T. bifasciatum, of food pellets treated with tissue and blood of P. nigra, vanadium complexes, and vanadium saltspounds were considered deterrent if the mean number of pellets eaten for any individual assay were less than or equal to 6 (P ≤ 0.043, Fisher exact test, one-tailed), as indicated by the …
Sea squirts accumulate vanadium compounds with potent antidiabetic activity, which are involved in immune defense. In this study, vanadium concentrations of fresh blood plasma, intestine, and muscle of the sea squirt Halocynthia roretzi were 6.3, 3.7 and 2.1 mg/kg respectively. Two vanadium binding proteins (VBPs) from blood plasma …
The blood cells of Ascidia gemmata, which is one of the vanadium accumulation ascidians, contain up to 350 mM vanadium [4], which corresponds to about 10 7 times the concentration in seawater [5], [6]. This unusual phenomenon observed in some ascidians has been studied by chemists, physiologists, and biochemists since its …
1. Introduction. Several families of ascidians (sea squirts or tunicates) accumulate extremely high levels of vanadium in their blood cells. The concentration …
Gel filtration analysis of vanadium in Ascidia nigra blood cell lysate. K. Gilbert K. Kustin Guy C. McLeod. Biology, Chemistry. Journal of cellular physiology. 1977; TLDR. Fractions from a Sephadex gel filtration of homogenized Ascidia nigra blood cell lysate were analyzed for vanadium by atomic absorption spectroscopy, and it was found that ...
advantage of gel filtration is that conditions can be varied to suit the type of sample or the requirements for further purification, analysis or storage without altering the separation. Gel filtration is well suited for biomolecules that may be sensitive to changes in pH, concentration of metal ions or co-factors and harsh environmental ...
The present study examined which of several types of blood cell contains this substance, using a combination of cell fractionation, chromatographies, and neutron activation analysis. The vanadium-containing blood cell type, so-called vanadocyte, is the signet ring cell in Ascidia sydneiensis samea as well as A. ahodori, and vanadobin was ...
This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.
To investigate the mechanism underlying this phenomenon, we performed an expressed sequence tag (EST) analysis of a complementary DNA library from vanadocytes of a vanadium-rich ascidian, Ascidia sydneiensis samea. We determined the nucleotide sequences of 1000 ESTs and performed a BLAST analysis against the SwissProt …
and the distribution and valence of vanadium in living Ascidia nigra blood cells. The study uses physical and chemical techniques which do not require lysis of the blood cells, e.g., nuclear magnetic resonance (Carlson, 1975). Degradative techniques are used only to obtain reliable quantitative data on the total vanadium content of the blood.
Gilbert K, Kustin K, McLeod GC (1977) Gel filtration analysis of vanadium in Ascidia nigra blood cell lysate. J Cell Physiol 93:309–311. Google Scholar Goodbody I (1974) The physiology of ascidians. Adv Mar Biol 12:1–149. Google Scholar Hall TH (1979) Biological X-ray microanalysis.
Search worldwide, life-sciences literature Search. Advanced Search Coronavirus articles and preprints Search examples: "breast cancer" Smith J
