Spatial optimization has been dedicated to the algorithms and methods for improving the spatial location science, applications of spatial. Cloud seeding strategic model. The Cloud Seeding decision-making process involves determining optimal locations for GBG and aircraft facilities to cover the target areas effectively.
Sequential Seeding Initialization for SNIC Superpixels 419 2.1 Grid Sampling Initialization For an image I = {Ii}W×H i=1, the 2-dimensional position coordinates in the Euclidean space and the 3-dimensional color intensity in the CIELAB color space of each pixel Ii can be denoted by p(Ii) = (x(Ii), y(Ii)) and c(Ii) = (l(Ii), a(Ii), b(Ii)), where W and H denote the …
Intensification of large-scale cell culture processes. In the past, the principal factor that contributed to boost titers was the integral of viable cell density (IVCD) [3].For years the IVCD has been increased by combining two strategies: first, by extending the growth phase by media optimization to achieve higher peak cells densities and …
Intensification of inoculation density can be used for titer improvement in fed-batch production culture. In order to research the effect of different inoculation densities and other related cell culture parameters on final product titer, fed-batch cell cultures for 3 mAbs expressed by 3 different CHO K1 GS cell lines were first evaluated using a design …
The secondary objective was the optimization of the cell seeding process on 3D-printed scaffolds using polycaprolactone (PCL) or polycaprolactone covered with tricalcium phosphate (PCL + 5% TCP).
Cell seeding into scaffolds plays a crucial role in the development of efficient bone tissue engineering constructs. Hence, it becomes imperative to identify the key factors that quantitatively predict reproducible and efficient seeding protocols. In this study, the optimization of a cell seeding process was investigated using design of experiments …
Seed loading is an important factor that will affect the kinetics of crystal growth and thereby affect the final product quality. The effect of seed loading with respect to the amount of seeds and the number of seed particles on the crystal size distribution of the final crystals in a continuous tubular crystalliser was reported b…
Request PDF | On Oct 16, 2020, Yang He and others published Seeding Techniques and Optimization of Solution Crystallization Processes | Find, read and cite all the research you need on ResearchGate
Process optimization is a set of methods and tools used to make processes more efficient and effective. There are several types of process optimization techniques, including process mapping, process mining, and Six Sigma. When optimizing a process, it's important to identify goals, analyze current processes, develop an optimized process, …
Over the past few decades, seeding strategies for solution crystallization processes have been developed for various applications, and significant improvements in crystal quality and process robustness have been achieved. Here a systematic review of seeding techniques from the preparation and characteristics of seed crystals to …
This research mainly focuses on the optimization of size distribution by controlling particle agglomeration during the oiling-out crystallization process in a model system of propyl paraben–ethanol–water. Nucleation control technologies, wet milling, seeding, and ultrasound were used to limit the agglomeration.
Seeding is a significant but challenging task for optimizing crystallization process operation, product quality, and process efficiency. With the development of modern crystallization technology, there have been considerable advancements in …
Process-modeling software permits more effective design and operation of crystallizers, and facilitates the optimization of seeding, operation profiling, and scaling up. For example, computational fluid dynamics (CFD) simulations help researchers to understand the hydrodynamics and crystallization kinetics that occur during the scale-up …
The two coupling strategies presented above enable hole seeding during the optimization process. In both cases, the design process consists of a handover from a pure density problem that does not need hole seeding but can produce intermediate density at convergence to a pure LS problem that relies on hole nucleation but features …
Seeding is a significant but challenging task for optimizing crystallization process operation, product quality, and process efficiency. With the development of modern …
A cell culture process adaptable to produce smallpox vaccine at large scale has been developed. To achieve this, Design of Experiments (DOE) was applied to identify and optimize critical cell culture process parameters for MRC-5 cell growth and recovery during cell expansion. For cell growth, a 25−1 partial factorial (two level, five factor, 16 …
These studies contribute to better understanding of the impact of seeding policies on crystal product quality and supported the fact that implementing seeding policy delivers stability to the crystallization process by suppressing secondary nucleation for the optimization of crystal size distribution (CSD).
The optimization of a cell seeding process was investigated using design of experiments (DOE) statistical methods and optimal conditions in terms of CSV and CSD were obtained when seeding cells in a regular scaffold with an excess of medium. Cell seeding into scaffolds plays a crucial role in the development of efficient bone tissue …
In this study, the optimization of a cell seeding process was investigated using design of experiments (DOE) statistical methods. Five seeding factors (cell type, scaffold type, seeding volume, seeding density, and seeding time) were selected and investigated by means of two response parameters, critically related to the cell seeding process ...
The secondary objective was the optimization of the cell seeding process on 3D-printed scaffolds using polycaprolactone (PCL) or polycaprolactone covered with tricalcium phosphate (PCL + 5% TCP).
Extended metabolite analysis in combination with flux balance analysis was applied for targeted optimization of High Seeding Density (HSD) CHO cell cultures. …
Cell seeding is the process of incorporating cells into or unto the scaffold prior to culture or implantation. ... Consequently, CFD simulation provides significant insight into the design and optimization of cell seeding devices while simultaneously saving time and resources. It is noteworthy, however, that little work has been done in ...
Abstract. The optimization of a batch cooling crystallizer has been traditionally sought with respect to the cooling profile and seeding characteristics that keep supersaturation at an optimum level throughout the operation. Crystallization processes typically have multiple performance objectives and optimization using different objective ...
The cell expansion process is a crucial part of generating cells on a large-scale level in a bioreactor system. Hence, it is important to set operating conditions (e.g. initial cell seeding distribution, culture medium flow rate) to an optimal level. Often, the initial cell seeding distribution factor is neglected and/or overlooked in the ...
The Cu reflow process sequence studied in this paper is shown in Fig. 1.As a 1 st step, a Cu PVD process with moderate wafer bias is conducted in a PVD Cu chamber to achieve suitable coverage in a feature before the subsequent Cu reflow step. In this Cu PVD chamber, an AC bias ranging from 0 to 1200 W can be applied to the …
The cell expansion process is a crucial part of generating cells on a large-scale level in a bioreactor system. Hence, it is important to set operating conditions (e.g. initial cell seeding distribution, culture medium flow rate) to an optimal level. Often, the initial cell seeding distribution fact …
@article{Pareek2023MultiobjectiveOO, title={Multi-objective optimization of seeding performance of a pneumatic precision seed metering device using integrated ANN-MOPSO approach}, author={C. M. Pareek and V. K. Tewari and Rajendra Machavaram}, journal={Eng. Appl. Artif.
Extended metabolite analysis in combination with flux balance analysis was applied for targeted optimization of High Seeding Density (HSD) CHO cell cultures. Flux balance analysis revealed distinct metabolic phases with high reactive oxygen species (ROS) formation and increased cell specific productivities. Based on the metabolic …
Optimization of the feeding strategy and the target seeding density was performed and interactions of the two parameters were assessed in order to enhance cell growth and MAb productivity. The feeding strategy was optimized to take into account daily process performance attributes and associated nutrient needs of the culture to maintain …
The secondary objective was the optimization of the cell seeding process on 3D-printed scaffolds using polycaprolactone (PCL) or polycaprolactone covered with tricalcium phosphate (PCL + 5% TCP). Biological evaluation of human ASC showed high efficiency of isolation obtaining a satisfying amount of homogeneous cell populations.
Process optimization is a set of methods and tools used to make processes more efficient and effective. There are several types of process optimization techniques, including process mapping, process mining, …
The potential of the proposed seeding distribution method as a useful tool to optimize the cell expansion process in modern bioreactor system applications is highlighted. Introduction In order to serve practical and commercial purposes (e.g. vaccine/blood production, tissue/organ engineering, cell therapies), the number of cells …
After the optimal seeding density has been determined, further process optimization should be done in the G-Rex6M well plate. These plates are part of the "M" series of devices, which incorporate the optimal media to gas-permeable membrane surface area ratio of 10 ml/cm 2 . At this ratio, the nutrient supply is sufficient to achieve the ...
Application of perfusion in the N-1 pre-stage bioreactor and a subsequent high seeding density (HSD) in the N-stage process has been shown to improve space–time yields in comparison to regular fed ... Based on the gathered data and metabolic analysis further targeted optimization strategies can be applied in the future …
4 Abstract Crystal seeding is the process of adding homogeneous or heterogeneous. 5 crystals to a crystallising solution to nucleate and/or grow more crystals. Seeding. 6 has emerged as one of …
